Chemistry of enzyme catalysis. Steady state enzyme kinetics. Transport kinetic methods.

Ligand binding and its application to enzymology. Kinetics of multiple binding sites.

Mechanisms of two substrate systems. Molecular models of allosterism. Enzyme models of

allosterism. Multi-enzyme complexes. Enzyme assays and techniques in enzymology.

Criteria for determining purity of enzymes. Enzyme reconstitution. Regulation of enzyme

activity and synthesis. -

Level of Study: 400 Level