Amino acids as building blocks of proteins; the peptide bond as covalent backbone of
proteins. Forces involved in the stabilization of protein structure. Protein isolation,
fractionation, purification and characterization. Amino acid analysis of peptides and proteins.
Methods for the determination of the sequence of amino acids in proteins. Molecular weight
determination of proteins. Techniques in protein biochemistry. Oxidative degradation of
amino acids and metabolism of one carbon units. Ammonia toxicity and urea formation.
Biosynthesis of amino acids and some derivatives; the urea cycle; metabolism of inorganic
nitrogen. Disorders of amino acid metabolism.