Enzyme histochemistry and its diagnostic application. The theory of stains and application, metallic impregnation and various histochemical methods. The dye theory. Properties of natural and synthetic dyes. Composition, preparation and storage of staining reagents. Testing of reagents. Common nuclear stain and counter stain for general tissue structures. Staining methods to demonstrate elastic, connective tissues and fibers. Toxicity of some reagents used as it applies to auto-radiography, electron microscopy and ultra microtomy. Suitable fixatives for use, processing techniques, impregnation/embedding and slide preparation/interpretation.



Level of Study: 500 Level