Fixation: Purpose and effect of fixative composition and uses of fixatives and their respective action on tissue components. Microscopic appearance of tissue after various methods of fixation. Function and scope of secondary fixation, post-fixation and post-mordantings. Knowledge of fixation of tissues for histochemical methods to include freeze drying and freeze drying substitutes. Decalcification – processing techniques – paraffin wax, embedding media for mechanical and manual processing. Microtomy-Microtomes (manipulation and uses of rocking, rotary, sledge, freezing, cryostat and ultra microtomes), knives – selection and maintenance for various microtomes, manual and mechanical sharpening. Section cutting (techniques used with different embedding media, attachment of sections to slides-frozen techniques method for rapid diagnosis).


Level of Study: 500 Level