Fixation: Purpose and effect of fixative composition and uses of
fixatives and their respective action on tissue components. Microscopic
appearance of tissue after various methods of fixation. Function and scope of
secondary fixation, post-fixation and post-mordantings. Knowledge of fixation
of tissues for histochemical methods to include freeze drying and freeze drying
substitutes. Decalcification – processing techniques – paraffin wax, embedding
media for mechanical and manual processing. Microtomy-Microtomes (manipulation
and uses of rocking, rotary, sledge, freezing, cryostat and ultra microtomes),
knives – selection and maintenance for various microtomes, manual and
mechanical sharpening. Section cutting (techniques used with different
embedding media, attachment of sections to slides-frozen techniques method for
rapid diagnosis).
- Lecturer: THEOPHILUS ERAMEH
Level of Study: 500 Level