Principles and techniques of isoelectric focusing. Protein separation of column chromatography. Finger printing, principles and techniques. Purification of proteins and enzymes. Ultracentrifugation and molecular weight determination. Culture of blood cells and parasite. Leucocyte typing, Platelet aggregation – principles and techniques. Radioisotopes in Haematology, Isotope labelling techniques, measurement of radioactivity, Fluorescent antibody techniques. Radioimmunoassay, ELIZA, Western blotting immunoelectrophoresis, Competitive protein binding. Automation in Haematology, Electrophoresis – starch agar gel and polymerase chain reaction. Cytochemical procedures. Lymphocyte Transformation Tests. Paul-Bunnel Test.


Level of Study: 500 Level