Analytical techniques, standardization and quality control. Validation of assay. Birth of a new method, devising new techniques. Biological trial and tests for acceptability. Solid/dry phase chemistry. Dipstick technology, thin film technology. Immobilized enzymes. Functional test in clinical chemistry. Liver function test. Renal function test. Gastrointestinal function test etc. Analytical techniques employed in qualitative and quantitative. Determination of (1) Enyzmes - Phosphatases, Transaminases, Dehydrogenases, Kinases (2) Hormones (3) Protein

– Total proteins, Albumin and Globulin specific protein (4) Lipids-cholesterol, triglycerides, glycerol, fatty acids and lipoproteins (5) Trace elements – Fe, Cu, Zn, Mg, Selenium (6) Non-protein nitrogen – Urea, Creatinine, Uric acid, Amino acids and Ammonia. Urinalysis, determination of urine specific gravity, osmolarity, qualitative tests for protein, glucose and reducing substances. Ketone bodies, bilirubin, urobilinogen and blood. Haemoglobin and its derivative in blood and urine. Chromatography, spectroscopy, spectrophotometry and photometry, AAS, Flame photometer, AES, Radioimmunoassay, ELISA AND EIA.


 


Level of Study: 500 Level