Methods for the demonstration of bacterial form and structure. Design and preparation of culture media. Sterilization and other methods of bacterial control. Aseptic procedures and methods for pure culture isolation, procedures for receiving, handling and processing of clinical specimens. Antibiotic assay, sensitivity test and chemotherapy. Plate reading. Principle and techniques of anaerobic bacteriology. Methods of total and viable counts. Stock culture preservation, quality control of culture and media. Record-keeping in Bacteriology laboratory. Staining techniques for spores, capsules and negative staining procedure, wet preparation, motility tests. Introductory Mycology.